Journal of bacteriology. 1998-02-01; 180.3: 746-8.

A new transformation-deficient mutant of Haemophilus influenzae Rd with normal DNA uptake

Gwinn ML, Ramanathan R, Smith HO, Tomb JF

PMID: 9457884


Haemophilus influenzae Rd is a gram-negative natural transformer. A mutant strain, RJ248, that has normal DNA uptake and translocation but whose transformation frequency is 300 times lower than that of wild-type H. influenzae and whose phage recombination is 8 times lower was isolated. The affected gene, comM, is induced during competence development in wild-type H. influenzae but not in RJ248.