Daniel Gibson, PhD


Daniel Gibson is an Professor in the Synthetic Biology group at the JCVI. Since joining the JCVI as a postdoc in 2004, Gibson led the JCVI efforts to synthesize two complete bacterial genomes. Those projects resulted in creation of the first synthetic bacterial cell and development of an enabling suite of DNA synthesis and assembly methods.

Prior to joining the JCVI, Gibson earned his PhD in Molecular Biology from the University of Southern California. While there, he used yeast as the model system for studying cell cycle surveillance mechanisms (checkpoints), which are significant in our understanding of cancer development. Before earning his Ph.D., he earned his Bachelor's degree in Biological Sciences from the State University of New York at Buffalo.


Gross Chromosomal Rearrangements in Kluyveromyces marxianus Revealed by Illumina and Oxford Nanopore Sequencing.
International journal of molecular sciences. 2020-09-26; 21.19:
PMID: 32993167
Direct transfer of a Mycoplasma mycoides genome to yeast is enhanced by removal of the mycoides glycerol uptake factor gene glpF.
ACS synthetic biology. 2019-01-15;
PMID: 30645947
One step engineering of the small-subunit ribosomal RNA using CRISPR/Cas9.
Scientific reports. 2016-08-04; 6.30714.
PMID: 27489041
Bacterial genome reduction using the progressive clustering of deletions via yeast sexual cycling.
Genome research. 2015-03-01; 25.3: 435-44.
PMID: 25654978
Synthetic biology: Construction of a yeast chromosome.
Nature. 2014-05-08; 509.7499: 168-9.
PMID: 24805340
Programming biological operating systems: genome design, assembly and activation.
Nature methods. 2014-05-01; 11.5: 521-6.
PMID: 24781325
Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing.
Nature protocols. 2014-04-01; 9.4: 743-50.
PMID: 24603933
Direct transfer of whole genomes from bacteria to yeast.
Nature methods. 2013-05-01; 10.5: 410-2.
PMID: 23542886
Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeast.
Nucleic acids research. 2012-11-01; 40.20: 10375-83.
PMID: 22941652
Assembly of large, high G+C bacterial DNA fragments in yeast.
ACS synthetic biology. 2012-07-20; 1.7: 267-73.
PMID: 23651249
Gene and genome construction in yeast.
Current protocols in molecular biology. 2011-04-01; Chapter 3.Unit3.22.
PMID: 21472698
Isolation of circular yeast artificial chromosomes for synthetic biology and functional genomics studies.
Nature protocols. 2011-01-01; 6.1: 89-96.
PMID: 21212778
Enzymatic assembly of overlapping DNA fragments.
Methods in enzymology. 2011-01-01; 498.349-61.
PMID: 21601685
Chemical synthesis of the mouse mitochondrial genome.
Nature methods. 2010-11-01; 7.11: 901-3.
PMID: 20935651
Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides.
Nucleic acids research. 2009-11-01; 37.20: 6984-90.
PMID: 19745056
Creating bacterial strains from genomes that have been cloned and engineered in yeast.
Science (New York, N.Y.). 2009-09-25; 325.5948: 1693-6.
PMID: 19696314
Enzymatic assembly of DNA molecules up to several hundred kilobases.
Nature methods. 2009-05-01; 6.5: 343-5.
PMID: 19363495
One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome.
Proceedings of the National Academy of Sciences of the United States of America. 2008-12-23; 105.51: 20404-9.
PMID: 19073939
Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.
Science (New York, N.Y.). 2008-02-29; 319.5867: 1215-20.
PMID: 18218864
Press Release

Venter Institute Scientists Create First Synthetic Bacterial Genome

Publication Represents Largest Chemically Defined Structure Synthesized in the Lab

Team Completes Second Step in Three Step Process to Create Synthetic Organism