Yong Xue, PhD

Staff Scientist

Yong Xue joined the J. Craig Venter Institute in Rockville, MD in 2018 in the Synthetic Biology and Bioenergy Group. His research interests involve expanding TAR cloning synthetic genomics tools, developed by Dr. Sanjay Vashee, to various viruses to facilitate vaccines development. He was also involved in developing broad range applications of synthetic microbiomes.

Prior to joining JCVI, Dr. Xue was an ORISE fellow at U.S. Food and Drug Administration where he worked on development of flow cytometric based rapid detection and enumeration platform of food-borne pathogens (RAPID-B). Dr. Xue holds a bachelor’s degree in microbiology from the Shandong University, a master’s degree in molecular microbiology from Virginia Tech, and a Ph.D. degree in molecular genetics from University of Arkansas at Little Rock.

Research Priorities

Signature infiltration and maintenance on plasmid elements creating a forensic microbial system
  • Design unique barcoded signatures and develop loop-mediated amplification (LAMP) and qPCR methods for rapid detection of signatures in field.
Elucidation of functions and genetic networks of HSV-1 virion proteins
  • Assemble HSV-1 genomes from part fragments with desired mutations.
  • Analyze protein interactions of HSV-1 virion proteins by GFP-trap, Western, and MS.
Generation and characterization of the SARS-CoV-2 infectious virus to facilitate therapeutic and vaccine development
  • Deconstruct SARS-CoV-2 genome and reassemble infectious clones with reporter genes.
  • Development of mouse adapted SARS-CoV-2 strains for in vivo studies.

Publications

The Effects of Different Degrees of Procyanidin Polymerization on the Nutrient Absorption and Digestive Enzyme Activity in Mice.
Molecules (Basel, Switzerland). 2018-11-08; 23.11:
PMID: 30413083
Synthetic Biology Approaches to the Sustainable Production of p-Coumaric Acid and Its Derivatives in Cyanobacteria.
Advances in experimental medicine and biology. 2018-01-01; 1080.261-277.
PMID: 30091099
Flow-Cytometry-Based Method to Detect Escherichia coli and Shigella Spp. Using 16S rRNA-Based Probe.
Current protocols in toxicology. 2017-02-01; 71.2.25.1-2.25.8.
PMID: 28146281
Development of a Flow Cytometry-Based Method for Rapid Detection of Escherichia coli and Shigella Spp. Using an Oligonucleotide Probe.
PloS one. 2016-02-25; 11.2: e0150038.
PMID: 26913737
Cyanobacteria as cell factories to produce plant secondary metabolites.
Frontiers in bioengineering and biotechnology. 2015-04-28; 3.57.
PMID: 25973419
Genetically engineering Synechocystis sp. Pasteur Culture Collection 6803 for the sustainable production of the plant secondary metabolite p-coumaric acid.
Proceedings of the National Academy of Sciences of the United States of America. 2014-07-01; 111.26: 9449-54.
PMID: 24927550
RpaA regulates the accumulation of monomeric photosystem I and PsbA under high light conditions in Synechocystis sp. PCC 6803.
PloS one. 2012-09-14; 7.9: e45139.
PMID: 23024802

Research Priorities

Signature infiltration and maintenance on plasmid elements creating a forensic microbial system
  • Design unique barcoded signatures and develop loop-mediated amplification (LAMP) and qPCR methods for rapid detection of signatures in field.
Elucidation of functions and genetic networks of HSV-1 virion proteins
  • Assemble HSV-1 genomes from part fragments with desired mutations.
  • Analyze protein interactions of HSV-1 virion proteins by GFP-trap, Western, and MS.
Generation and characterization of the SARS-CoV-2 infectious virus to facilitate therapeutic and vaccine development
  • Deconstruct SARS-CoV-2 genome and reassemble infectious clones with reporter genes.
  • Development of mouse adapted SARS-CoV-2 strains for in vivo studies.