Botulinum Neurotoxin (BoNT) Resource Guide

BoNT Protocols

Biosafety Protocols   Epitope Protocols   Reagent Links   Safety Requirements

Biosafety Protocols

• Biosafety Resources at the Animal & Plant Health Inspection Service (APHIS)
• CDC: Emergency Preparedness & Response Information
  • Specimen Collection/ Lab Testing
  • Sentinel Laboratory Guidelines  
• CDC's Biosafety in Microbiological and Biomedical Laboratories (BMBL) 4th Edition  
  • Section VII: Agent Summary Statement for Clostridium botulinum
• Department of Transportation: Transportation requirements for infectious substances  
• FDA's Bacteriological Analytical Manual Online. Chapter 17: Clostridium botulinum
• Summary of the NIAID expert panel on Botulinum diagnostics   
• USAMRIID Blue Book: (Botulinum Toxin, page 81)  
  • Appendix G: BW Agent Laboratory Identification Protocols 

Epitope Protocols

• Epitope Tagging Manual  at Roche Applied Science

Reagent Links

• Linscott's Directory of Immunological and Biological Reagents
• NIAID Reference Reagent Repository

Safety Requirements: Working with Botulinum Toxin

• CDC FORM EA101 - Guidance document for report of transfer of select biological agents and toxins  
• EA-101 Instructions  
• Laboratory Guidelines for Botulinum toxin by the American Society of Microbiology  
• Lindstrom M and H Korkeala. 2006: Laboratory diagnostics of botulism  
• Lewis KH and EV Hill. 1946: Practical Media and Control Measures for Producing Highly Toxic Cultures of Clostridium

• FBI Background Check Forms

  • National Instant Criminal Background Check System
  • FBI Identification Record Request
  • Example Letter Requesting a criminal background check 

• Health Protection Agency (HPA) Forms

  • Botulism questionnaire