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Entamoeba Metabolism Enzymes

      

Entamoeba Metabolism Enzymes (section 4)

Metabolism Enzymes (section 4.1)

Review of major energy generating and biosynthetic metabolism enzymes was highlighted in the Advances in Parasitology Review (submitted): Structure and Content of the Entamoeba histolytica Genome. The supplementary table data, presented here corresponds to section 4.1 of this review. The metabolism enzymes data is presented by section 4.1 sub-sections with Section Number, Section Name, EC Link to the IUBMB Enzyme Nomenclature Database, KEGG Link to the KEGG database (Kyoto Encyclopedia of Genes and Genomics), Protein Accession to NCBI's Entrez Protein database, Model Protein Accession (XP) to NCBI's Entrez Protein database, mRNA models (XM) included in the reannotation linked to TIGR's Annotation Display (Pathema Annotation Link) for E. histolytica annotation, or mRNA models not included in the reannoation linked to NCBI's Nucleotide database (Models Excluded from the Reannotation), Gene Name and Comment.

Footnote:
Entries with * in the Protein Accession column indicates when one or more of the genome project models was found to be identical to the old published sequences (Protein Accession). To indicate this situation, the Protein Accession is included in the same row with each highly similar model.
Please note that entries with ** have multiple Pathema Annotation.
*** The seven annotated purine nucleoside phosphorylase (PNP) genes fall into two groups: the short group with 4 members and the long group with 3 members. The short and long groups share ≤ 25% protein sequence identity.

Section Number Section Name EC Link KEGG Link Protein Accession Model Protein Accession Pathema Annotation Link Models Excluded from the Reannotation Gene Name Comment
4.1.1.1 Hexokinase 2.7.7.1 2.7.7.1 CAA57681 XP_653620 EHI_098290   Hxk1  

      CAA57682 XP_655965 EHI_098560   Hxk2  
4.1.1.2 Glucose-6-phosphate isomerase 5.3.1.9 5.3.1.9   XP_650595   XM_645503
 

        XP_650607   XM_645515
 
4.1.1.3 Phosphofructokinase-60kDa 2.7.1.90 2.7.1.90 AAC04465 XP_653173 EHI_000730  
 

Phosphofructokinase-48kDa **     CAA57659 XP_653373 EHI_103590  
 

      2105198A XP_653373 EHI_103590  
 

        XP_653373 EHI_103590  
 

        XP_653373 EHI_103590  
 

        XP_653723 EHI_163630  
 

        XP_656482 EHI_187040  
 
4.1.1.4 Fructose-1,6-bisphosphate aldolase 4.1.2.13 4.1.2.13 AAL23716 XP_650373 EHI_098570  
 

        XP_655966    
 
4.1.1.5 Triose-phosphate isomerase 5.3.1.1 5.3.1.1 CAA73817 XP_650725 EHI_056480  
 
4.1.1.6 Glyceraldehyde-3-phosphate dehydrogenase 1.2.1.12 1.2.1.12 AAA29100* XP_650356 EHI_008200  
 

      AAA29100* XP_654359 EHI_008200  
 

      AAA29100* XP_656419 EHI_008200  
 

        XP_648981   XM_643889
 

        XP_650370   XM_645278
 
4.1.1.7 Phosphoglycerate kinase 2.7.2.10 2.7.2.10   XP_653686 EHI_188180  
 
4.1.1.8 Phosphoglycerate mutase 5.4.2.1 5.4.2.1   XP_649031   XM_643939
 

        XP_654182 EHI_050940  
 

        XP_649053 EHI_103650  
 

        XP_651808 EHI_129820  
 

        XP_657284 EHI_093870  
 
4.1.1.9 Enolase ** 4.2.1.11 4.2.1.11 P51555 XP_649161 EHI_130700  
 

        XP_649161 EHI_130700  
 

        XP_649161 EHI_130700  
 

        XP_648185   XM_643093
Truncated  
4.1.1.10 Pyruvate, orthophosphate dikinase 2.7.9.1 2.7.9.1 P37213* XP_657332 EHI_009530  
 

      AAA19026*      
 

      P37213* XP_654666   XM_649574


      AAA19026*      


        XP_657250   XM_652158


        XP_657251   XM_652159


Pyruvate kinase ** 2.7.1.40 2.7.1.40   XP_653635   XM_648543


        XP_648240   XM_643148


        XP_649089 EHI_098420  


        XP_649089 EHI_098420  

4.1.1.11 Pyruvate: ferredoxin oxidoreductase ** 1.2.7.1 1.2.7.1 CAA90575* XP_652984   XM_647892


      AAB49653*      


      CAA90575* XP_657019 EHI_051060  


      AAB49653*      


        XP_657019 EHI_051060  


        XP_657019 EHI_051060  


Ferredoxin     P11425* XP_655183   XM_650091


      P11425* XP_655182   XM_650090


        XP_654311   XM_649219


        XP_652694   XM_647602


        XP_653679   XM_648587


        XP_651284   XM_646192


        XP_652210   XM_647118

4.1.1.12 Acetyl-CoA synthetase 6.2.1.13 6.2.1.13 AAF88064 XP_656290 EHI_178960  
 
4.1.1.13 Alcohol dehydrogenase 1 ** 1.1.1.2 1.1.1.2 P35630* XP_653477   XM_648385 ADH1 NADPH dependent  

    P35630* XP_653507 EHI_023110   ADH1  

        XP_653507 EHI_023110  
 

        XP_653507 EHI_023110  
 

      P35630* XP_655661   XM_650569 ADH1  

        XP_652772 EHI_107210   ADH1 Divergent  

Bifunctional alcohol / aldehyde dehydrogenase 1.1.1.1 1.1.1.1 Q24803* XP_649240   XM_644148 ADH2 NADPH dependent  

  1.2.1.10 1.2.1.10 S53319*      
 

      Q24803* XP_650966   XM_645874 ADH2  

      S53319*      
 

      Q24803* XP_652300   XM_647208 ADH2  

      S53319*      
 

      Q24803* XP_655817   XM_650725 ADH2  

      S53319*      
 

      Q24803* XP_655843 EHI_150490   ADH2  

      S53319*      
 

      Q24803* XP_648290   XM_643198 ADH2 Truncated  

      S53319*      
 

Alcohol dehydrogenase 3 ** 1.1.1.2 1.1.1.2   XP_649070 EHI_088020   ADH3 NADH dependent

      Q24857* XP_649262 EHI_198760   ADH3  

      S68143*      
 

      Q24857* XP_649823   XM_644731 ADH3  

      S68143*      
 

      XP_650419 EHI_125950   ADH3

        XP_650419 EHI_125950  
 

        XP_650419 EHI_125950  
 

        XP_653023   XM_647931 ADH3  

      Q24857* XP_653772   XM_648680 ADH3  

      S68143*      
 

        XP_653883 EHI_192470   ADH3  

      Q24857* XP_654995 EHI_198760   ADH3  

      S68143*      
 

      Q24857* XP_655172   XM_650080 ADH3  

      S68143*      
 

        XP_655409     ADH3  

        XP_656521     ADH3  

Other alcohol dehydrogenases ** 1.1.1.- 1.1.1.-   XP_656535 EHI_042260  
Zn containing?  

        XP_652753 EHI_000410  
Fe containing?  

      XP_652262 EHI_166490  
 

        XP_652262 EHI_166490  
 

Aldehyde dehydrogenase 1.2.1.- 1.2.1.- AAA19741 XP_656548 EHI_042140   ALDH1 NADPH dependent  
4.1.2 Glycogen phosphorylase 2.4.1.1 2.4.1.1 AAL23578* XP_656847 EHI_138380  
 

      AAN17338* XP_655667 EHI_096830  
 

      AAL23578* XP_655151   XM_650059
 

      AAL23578* XP_655150   XM_650058
 

      AAL23578* XP_655120 EHI_138380  
 

      AAN17338* XP_651019   XM_645927
 

Glycogen debranching enzyme ** 3.2.1.33 3.2.1.33   XP_653608 EHI_098360  
 

  2.4.1.25 2.4.1.25        
 

        XP_653608 EHI_098360  
 

        XP_653608 EHI_098360  
 

        XP_648736   XM_643644
Truncated  

Phosphoglucomutase 5.4.2.2 5.4.2.2 CAA74796 XP_657021 EHI_110120   PGM  

Phosphoglucomutase/phosphomannomutase family **       XP_650107 EHI_084950  
 

        XP_650107 EHI_084950  
 

        XP_648671 EHI_185280  
 

Glycogen synthase 2.4.1.11 2.4.1.11   XP_653076 EHI_085980  
 

Glycogen branching enzyme 2.4.1.18 2.4.1.18   XP_653706   XM_648614
 

        XP_648487 EHI_106090  
Truncated  

UTP--glucose-1-phosphate uridylyltransferase 2.7.7.9 2.7.7.9   XP_652750 EHI_000440  
 

UTP--N-acetyl-glucosamine-1-phosphate uridyltransferases       XP_655359 EHI_021200  
 

        XP_651966 EHI_039830  
 
4.1.3              
 
4.1.3.1 Fructokinase 2.7.1.4 2.7.1.4   XP_652087 EHI_054510  
 

Galactokinase 2.7.1.6 2.7.1.6   XP_649045 EHI_094100  
 

UDP-glucose 4-epimerase 5.1.3.2 5.1.3.2   XP_650346 EHI_192050  
 
4.3.1.2 Aldose 1-epimerase 5.1.3.3 5.1.3.3 AAW29083 XP_649046 EHI_094090  
 
4.3.1.3 Ribokinase 2.7.1.15 2.7.1.15   XP_655903 EHI_138020  
 

Xylulokinase 2.7.1.17 2.7.1.17   XP_656185 EHI_012230  
 
4.3.1.4 Transketolase 2.2.1.1 2.2.1.1   XP_650836   XM_645744
 

        XP_650850 EHI_002160  
 

        XP_651488   XM_646396
 

        XP_648840 EHI_011410  
Truncated  

        XP_648680   XM_643588
Truncated  

        XP_651838    
Truncated

        XP_651839    
Truncated
4.2 Aminotransferase ** 2.6.1.- 2.6.1.-   XP_653431 EHI_006080  


      XP_655090 EHI_084670  


        XP_655090 EHI_084670  


        XP_655099   XM_650007


Alanine aminotransferase 2.6.1.2 2.6.1.2   XP_655674 EHI_096750  


Branched-chain amino acid aminotransferase 2.6.1.42 2.6.1.42   XP_656980 EHI_152310  

4.2.1 Asparaginase 3.5.1.1 3.5.1.1   XP_656293 EHI_178920  


        XP_656232   XM_651140


        XP_649119 EHI_178920  


        XP_656586 EHI_050740  


Aspartase 4.3.1.1 4.3.1.1   XP_655826   XM_650734


Fumarase 4.2.1.2 4.2.1.2   XP_650763 EHI_117270  


Malate dehydrogenase 1.1.1.37 1.1.1.37   XP_656785 EHI_067860  


        XP_655679 EHI_152670  


        XP_655601 EHI_092450  


        XP_655084   XM_649992


        XP_650866   XM_645774


        XP_650855   XM_645763


        XP_649756 EHI_165350  


        XP_648568   XM_643476


Malic enzyme 1.1.1.39 1.1.1.39 AAF43042 XP_648590 EHI_044970  


        XP_657198   XM_652106

4.2.2 Threonine dehydratase 4.3.1.19 4.3.1.19   XP_650405   XM_645313


        XP_652480 EHI_126080  


        XP_655614    


        XP_657171 EHI_049910  


D-glycerate dehydrogenase 1.1.1.29 1.1.1.29 BAD00049* XP_648124   XM_643032


      BAD00049* XP_655428   XM_650336


D-glycerate kinase 2.7.1.31 2.7.1.31   XP_650055 EHI_196910  


        XP_653159 EHI_196910  

4.2.3 Methionine γ-lyase 4.4.1.11 4.4.1.11 BAC75877* XP_650718   XM_645626 MGL1

      BAC75877* XP_648767 EHI_144610   MGL1  

      BAC75877* XP_652096 EHI_144610   MGL1  

      BAC75878 XP_648806 EHI_142250   MGL2  

S-adenosylmethionine synthetase 2.5.1.6 2.5.1.6   XP_648875 EHI_174250  
 

        XP_650698 EHI_174250  
 

        XP_652506 EHI_174250  
 

        XP_652854   XM_647762
 

        XP_653872   XM_648780
Truncated  

        XP_653839   XM_648747
Truncated  

        XP_653840   XM_648748
Truncated  

S-adenosylhomocysteine hydrolase ** 3.3.1.1 3.3.1.1   XP_651721   XM_646629
 

        XP_651935 EHI_068250  
 

        XP_651935 EHI_068250  
 

        XP_651935 EHI_068250  
 

        XP_654136    
Truncated  

5’-methylthioadenosine / S-adenosyl homocysteine nucleosidase 3.2.2.9 3.2.2.9   XP_653171 EHI_000720  
 

Aspartate aminotransferase 2.6.1.1 2.6.1.1        
 
4.2.4 Arginase 3.5.3.1 3.5.3.1   XP_656978 EHI_152330  
 
4.2.5 Glucosamine-fructose-6-phosphate aminotransferase 2.6.1.16 2.6.1.16   XP_650078 EHI_029560  
 
4.2.6 Tryptophanase 4.1.99.1 4.1.99.1   XP_649067 EHI_111610  
 
4.2.7 Alanine aminotransferase 2.6.1.2 2.6.1.2   XP_655674 EHI_096750  
See 4.2  
4.2.8 Histidine ammonia-lyase ** 4.3.1.3 4.3.1.3   XP_651572 EHI_036510  
 

        XP_651572 EHI_036510  
 

Ornithine/arginine/lysine? decarboxylase 4.1.1.- 4.1.1.-   XP_648734 EHI_098380  
 
4.3 Ornithine decarboxylase 4.1.1.17 4.1.1.17 AAX35675 XP_655113 EHI_100430  
 

Arginase/Agmatinase?       XP_656978 EHI_152330  
 
4.4.1 Cysteine synthase 2.5.1.47 2.5.1.47 BAA21916 XP_652277   XM_647185 Cys synthase 1  

      BAA22144 XP_648291   XM_643199 Cys synthase 2  

      BAE19925 XP_653246 EHI_060340   Cys synthase 3  

      BAA21915* XP_649241   XM_644149
 

      BAA21915* XP_650965 EHI_024230  
 

        XP_653106   XM_648014
Truncated  

        XP_653109   XM_648017
Truncated  

Serine acetyl transferase 2.3.1.30 2.3.1.30 BAA82868* XP_650765    
SAT1

      BAA82868* XP_651909     SAT1  

      BAE19927 XP_650001 EHI_021570   SAT2  

      BAE19928 XP_656373 EHI_153430   SAT3  

D-phosphoglycerate dehydrogenase 1.1.1.95 1.1.1.95   XP_652157   XM_647065
 

Phospho-L-serine aminotransferase ** 2.6.1.52 2.6.1.52   XP_655383 EHI_026360  
 

        XP_655383 EHI_026360  
 

O-phospho L-serine phosphatase 3.1.3.3 3.1.3.3        
 
4.4.2 Glutamine synthase 1.4.1.13 1.4.1.13   XP_648656 EHI_095110  
 

        XP_649804   XM_644712
 

        XP_653475   XM_648383
 

        XP_655732   XM_650640
 

        XP_655790 EHI_155520  
 

Glutamate synthase 6.3.1.2 6.3.1.2   XP_657304   XM_652212
 

        XP_656997 EHI_110520  
 

        XP_653573 EHI_045340  
 

Aspartate ammonia ligase 6.3.1.1 6.3.1.1   XP_657492 EHI_148470  
 
4.4.3 Glutamate dehydrogenase 1.4.1.2 1.4.1.2   XP_648864   XM_643772
 
4.5.1.1 Isopentenyl-diphosphate delta-isomerase 5.3.3.2 5.3.3.2   XP_654698 EHI_194410  
 

Prenyl transferases 2.5.1.- 2.5.1.-   XP_649270    
 

        XP_650479   XM_645387
 

        XP_650913    
 

        XP_653910   XM_648818
 

        XP_655958   XM_650866
 

Protein farnesyl transferase 2.5.1.58 2.5.1.58 BAC98941 XP_649581 EHI_074760  
Alpha chain  

      BAC98942 XP_653441 EHI_006190  
Beta chain  

Protein geranylgeranyl transferase 2.5.1.59 2.5.1.59 BAE72105* XP_654936 EHI_077230  
Beta chain  

      BAE72105* XP_654952   XM_649860
Beta chain  

Protein (Rab-) geranylgeranyltransferase II ** 2.5.1.60 2.5.1.60 BAE72106 XP_655655 EHI_023180  
Alpha chain  

        XP_655655 EHI_023180  
 

      BAE72107 XP_648235 EHI_025110  
Beta chain  

CAAX prenyl proteases 3.4.24.84 3.4.24.84   XP_648770 EHI_075660  
 

        XP_656466 EHI_187200  
 

Prenylcysteine carboxyl methyltransferase ** 2.1.1.100 2.1.1.100   XP_653433 EHI_006100  
 

        XP_657326 EHI_045150  
 

        XP_657326 EHI_045150  
 
4.5.1.2 Acetyl-CoA carboxylase       XP_651829 EHI_082530  
 

Fatty acid elongase       XP_650157 EHI_158240  
 

        XP_650821   XM_645729
 

        XP_654548   XM_649456
Truncated  

        XP_654571 EHI_092190  
 

        XP_656100 EHI_112870  
 

        XP_656160   XM_651068
Truncated  

        XP_654403 EHI_111000  
 

        XP_654651 EHI_009370  
 
4.5.2.1 Glycerol kinase 2.7.1.30 2.7.1.30   XP_655213 EHI_164850  
 

1-acylglycerol-3-phosphate O-acyltransferase 2.3.1.15 2.3.1.15   XP_655766 EHI_155730  
 

        XP_649422 EHI_097610  


Phosphatidate phosphatase 3.1.3.4 3.1.3.4   XP_650970 EHI_024410  


Choline/Ethanolamine kinase 2.7.1.82 2.7.1.82   XP_656977 EHI_152340  


  2.7.1.32 2.7.1.32   XP_657480 EHI_148580  


Choline/Ethanolamine-phosphate cytidylyltransferase 2.7.7.15 2.7.7.15   XP_649803 EHI_095120  


  2.7.7.14 2.7.7.14        


        XP_654185 EHI_140590  


Ethanolamine phosphotransferase/ Diacylglycerol cholinephosphotransferase ** 2.7.8.1 2.7.8.1        


  2.7.8.2 2.7.8.2   XP_650432    


        XP_651514 EHI_182040  


        XP_651757    


        XP_652068 EHI_054720  


        XP_652068 EHI_054720  


        XP_653595 EHI_045570  


      XP_654296 EHI_099290  


        XP_654296 EHI_099290  


        XP_654296 EHI_099290  


      XP_654313 EHI_080720  


        XP_654313 EHI_080720  


        XP_654313 EHI_080720  


        XP_656778 EHI_067920  


Phosphatidate cytidylyltransferase 2.7.7.41 2.7.7.41   XP_652071 EHI_054750  


        XP_654029   XM_648937


        XP_655878   XM_650786


Phosphatidylserine synthase 2.7.8.8 2.7.8.8   XP_657102 EHI_009800  


Unidentified methyltransferases       XP_649121 EHI_178940  


        XP_650079 EHI_029570  


        XP_653353 EHI_103830  


        XP_656230   XM_651138


L-myo-inositol 1-phosphate synthase 5.5.1.4 5.5.1.4 CAA72135* XP_656291 EHI_178940  


      CAA72135* XP_649764   XM_644672


      CAA72135* XP_652695    


        XP_654310   XM_649218


Phospholipid-cholesterol acyltransferase 2.3.1.43 2.3.1.43   XP_652402 EHI_065250  


        XP_654024 EHI_064540  


        XP_654094 EHI_031360  


        XP_654285 EHI_099180  


        XP_654915    


        XP_655259 EHI_136400  


        XP_656149 EHI_035750  


Phospholipase A **       XP_650745 EHI_050610  


        XP_651968   XM_646876


        XP_652675 EHI_200740  


        XP_653376 EHI_103880  


        XP_653376 EHI_103880  


        XP_653645 EHI_065690  


        XP_654790 EHI_167000  


        XP_655078 EHI_014360  


        XP_656377 EHI_153390  


        XP_656598   XM_651506


        XP_656644 EHI_105850  


        XP_656691 EHI_073330  


Phospholipase D ** 3.1.4.4 3.1.4.4   XP_649037 EHI_146550  


        XP_649913    


        XP_651826 EHI_082560  


        XP_651826 EHI_082560  


Phosphatidylinositol-specific phospholipase C ** 3.1.4.11 3.1.4.11   XP_655339   XM_650247


        XP_655853    


        XP_655853    


        XP_655853    

4.7 Aspartate transcarbamoylase 2.1.3.2 2.1.3.2   XP_650972 EHI_024420  


Cytidylate kinase 2.7.4.14 2.7.4.14   XP_652359 EHI_143580  


Dihydroorotate dehydrogenase 1.3.99.11 1.3.99.11   XP_656317 EHI_012980  


Dihydropyrimidinase 3.5.2.2 3.5.2.2   XP_650972 EHI_024420  


Dihydropyrimidine dehydrogenase 1.3.1.2 1.3.1.2   XP_656317 EHI_012980  


Ribose phosphate pyrophosphokinase 2.7.6.1 2.7.6.1   XP_656141 EHI_035650  


Thymidylate kinase 2.7.4.9 2.7.4.9   XP_654383 EHI_008040  


PRPP synthetase 2.7.6.1 2.7.6.1   XP_652567 EHI_008460  


Uridine Kinase 2.7.1.48 2.7.1.48   XP_651299 EHI_051730  


Thioredoxin Reductase ** 1.6.4.5 1.6.4.5   XP_655748 EHI_155440  


  1.6.4.5 1.6.4.5   XP_655748 EHI_155440  


  1.6.4.5 1.6.4.5   XP_648818   XM_643726


AMP Deaminase 3.5.4.6 3.5.4.6   XP_652654 EHI_029100  


Purine nucleoside phosphorylase (PNP) *** 2.4.2.1 2.4.2.1   XP_655553 EHI_130960  
Short PNP group

        XP_655550 EHI_130930  
Short PNP group

        XP_655507   XM_650415
Short PNP group

        XP_655250   XM_650158
Short PNP group

        XP_655398 EHI_200080  
Long PNP group

        XP_654874 EHI_012720  
Long PNP group

        XP_652740 EHI_048740  
Long PNP group

Nucleoside diphosphate kinase 2.7.4.6 2.7.4.6   XP_655004 EHI_104360  
 
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